Metal Cation/anion Speciation via Paired-ion, Reversed Phase Hplc with Refractive Index And/or Inductively Coupled Plasma Emission Spectroscopic Detection Methods

Conventional high performance 1 i qu i d chromatography instrumentation and packing materials can be inexpensively and rapidly utilized for the qualitative and quantitative analysis of various metal cations or anions. The final approaches utilize reversed phase HPLC in the form of paired-ion separations. The detection of individually eluted, fully resolved metal cations or anions is possible conventional refractive index or inductively coupled plasma emission spectroscopic detection. In many cases, unresolved mixtures of metal cations, eluted as a single peak on HPLC, can be resolved and identified via the use of ICP detection. Both metal cations and anions can be easily resolved, according to oxidation states, using paired-ion techniques, in combination with ICP detection. Final data representation can be in the form of conventional, continuous RI and/or ICP chromatograms, pulsed data ICP presentations, and/or tabular ICP data presentation. INTRODUCTION (1) Inorganic metal toxicity has long been an area of intense biological, toxicological, and medical interest. The apparent toxic properties of most, * Author to whom correspondence and reprint requests should be addressed. 463 J R L F I I C R T R P . ( ), 463-478 (1982) I / I A ION I I O , I I IND / IN IVELY P SM I ION IC IO E * D. 1 5 r l tute ic nal si ort n niversi unti on v st a sachuset . . . it nalyt trum i i o trum t ion or t n il i t a sachusett nvent l g form liq o t aphy trum t ion ng ateri n y t ize l ive anti ive l o et o i in l s t ize sed i o ion . ion d ly e . y ve et l ion ons s via t ive n t y ed asm i on rosco c t a r i t et io e , v t ied h C t o t et o n y ve ng i ion i ion ech q . n bi t on i C t o i t ion n h orm enti l. inuous / C at a via ed a CP t ion . / via l C t r sentati n.--NTROD ION ) n g c et y ong e i l i l o , edi r ert ost, ut o m respondence r